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Introducing plexWell™ ...

The Challenge of Large-Scale NGS Library Preparation

Most benchtop sequencing instruments are idle 90% of the time due to high running costs and/or a shortage of “high value” samples. As a consequence, significant productivity increases and economic benefits offered by next-generation sequencing (NGS) have been unrealized by many fields of molecular biology and clinical research where cost-effective sequencing of 100s to 1000s of kilobase-scale templates (for example, long PCR amplicons, plasmids, bacterial genomes, etc.) would be highly desirable.

plexWell™ Library Preparation Technology

seqWell’s new Illumina-compatible plexWell Library Preparation technology (patent pending) permits an order-of-magnitude improvement in the number of samples that can be prepared and readily sequenced on benchtop NGS instruments. This means that the total NGS cost per sample is lowered dramatically (by 10X or more) by spreading the cost of sequencing over 100s – 1000s of samples. At launch, the plexWell product will be offered in at least two kit configurations: for 384 and 1,536 samples.

plexWell™ technology adds to the research toolbox by overcoming two major obstacles to greater NGS utilization: total NGS cost-per-sample and streamlining the library preparation workflow.

How does plexWell™ work?

Technology Summary

At the core of our novel plexWell™ technology is a new approach to making NGS libraries that rethinks how library prep works for large numbers of samples. Instead of making individual libraries out of many different samples and then pooling for sequencing, the plexWell workflow allows up to 96 libraries to be prepared as a single pool after a simple molecular tagging step.  This dramatically reduces the workflow (time) and material (cost) needed to prepare libraries for a 96-well plate.


plexWell Applications

A Versatile Tool

plexWell is particularly suited for applications needing up to approximately 1 gigabase of raw sequence data, for one or more 96-well plates of template DNA.  Examples shown below include full-length plasmid sequencing, multi-kb amplicon sequencing, and small (microbial) genome sequencing. Depending on the target length of the templates, sequencing can be optionally performed on an Illumina® MiSeq or NextSeq500 benchtop sequencer.

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plexWell Pro Services

We are currently offering plexWell Pro — our in-house professional sequencing service that provides access to plexWell technology for interested customers. With plexWell Pro service, we perform all library preparation, sequencing and data processing on site, along with customized bioinformatics support. Our team of sequencing specialists has extensive NGS experience, and we would be happy to discuss whether your project is a good fit for the plexWell technology.


The plexWell Early Access Program

We are seeking external partners and researchers who are interested in testing plexWell™ library preparation kits. Suitable template types include plasmids, BACs, and amplicons from any source, including bacterial colonies and human DNA. Any input DNA up to the size of a large bacterial genome (e.g., 10 Mb) can be sequenced using the plexWell beta-test kit. One requirement is that the DNA in each well is clonal (not a complex mixture of samples)


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