The Challenge of Large-Scale NGS Library Preparation
Most benchtop sequencing instruments are idle 90% of the time due to high running costs and/or a shortage of “high value” samples. As a consequence, significant productivity increases and economic benefits offered by next-generation sequencing (NGS) have been unrealized by many fields of molecular biology and clinical research where cost-effective sequencing of 100s to 1000s of kilobase-scale templates (for example, long PCR amplicons, plasmids, bacterial genomes, etc.) would be highly desirable.
plexWell™ Library Preparation Technology
seqWell’s new Illumina-compatible plexWell Library Preparation technology (patent pending) permits an order-of-magnitude improvement in the number of samples that can be prepared and readily sequenced on benchtop NGS instruments. This means that the total NGS cost per sample is lowered dramatically (by 10X or more) by spreading the cost of sequencing over 100s – 1000s of samples. At launch, the plexWell product will be offered in at least two kit configurations: for 384 and 1,536 samples.
At the core of our novel plexWell™ technology is a new approach to making NGS libraries that rethinks how library prep works for large numbers of samples. Instead of making individual libraries out of many different samples and then pooling for sequencing, the plexWell workflow allows up to 96 libraries to be prepared as a single pool after a simple molecular tagging step. This dramatically reduces the workflow (time) and material (cost) needed to prepare libraries for a 96-well plate.
A Versatile Tool
plexWell is particularly suited for applications needing up to approximately 1 gigabase of raw sequence data, for one or more 96-well plates of template DNA. Examples shown below include full-length plasmid sequencing, multi-kb amplicon sequencing, and small (microbial) genome sequencing. Depending on the target length of the templates, sequencing can be optionally performed on an Illumina® MiSeq or NextSeq500 benchtop sequencer.